It had been showed that osteoporosis in postmenopausal women bring on locomotive syndrome (locomo) which are restricted in one's ability to walk owing to a motor dysfunction. Besides, menopausal disorders symptoms such as fatigue might cause exercising less and aggravate locomo. In this study, we examined effects of novel women's preservative herbal medicine on symtoms like locomo of ovariectomized (OVX) mice.
The female ICR mice of 10-weeks old were received ovariectomy or sham operation. The mixed diet of herbal medicine (1 % or 2 %) were ingested to each groups for 5 weeks starting from 8 weeks after OVX. The spontaneous activity of the mice was evaluated by using an activity sensor at dark term (19:00-7:00). At the end of study period, animals were autopsied and bone and soleus muscle were collected for micro-CT, bone strength and gene expression study.
In the result, herbal medicine (2%) significantly suppressed decreased the spontaneous activity in dark term of OVX model mice. Additionally, the decrease in soleus muscle weight per body weight and bone strength due to OVX was improved by ingestion of herbal medicine (2 %). Moreover tartrate-resistant acid phosphatase (TRAP) and TNF receptor-associated factor 6 (TRAF6) expression level involved in bone resorption were increased by OVX and significantly recovered by herbal medicine (2 %). The present result suggested that this herbal medicine improved condition of reduced mobility mobility due to impairment of bones and muscles induced OVX.