We recently reported that enriched environment (EE) was significantly decreased voiding frequency and total voided volume in BALB/c female mice. However, the mechanism is unknown. In this study, we investigated whether temozolomide, a neurogenesis inhibitor, and PCPA (p-chlorophenylalanine), a serotonin synthetic enzyme inhibitor, were affected micturition function in mice with EE. Temozolomide was injected in BALB/c female mice on 3 days every week for a total of 6 weeks. The mice were reared in the EE or standard environment (SE) from the day after the end of temozolomide administration. PCPA (100 mg/kg, i.p., administered for 4 days) was injected in mice between 22 and 23 weeks from the start of EE. Micturition activity of freely moving mice was measured the day after the last day of PCPA administration. In the light period, EE was significantly decreased in voiding frequency and total voided volume compared to SE. Temozolomide was significantly increased in voiding frequency and total voided volume in the EE group at the light period. In the dark period, PCPA was significantly decreased in total voided volume, mean voided volume, voiding duration, and maximum flow rate, regardless of the rearing environment. These results suggested that neurogenesis may be related to the enrichment effects on micturition function.