Maternal obesity is reported to affect the offspring outcomes, such as increasing the risk of psychiatric disorders and metabolic diseases. In this study, we investigated whether maternal obesity affects nurturing behavior in mice. Eight-week-old female C57BL/6J mice were fed with a high-fat diet (HFD) or a regular diet (RD) for 28 days, and then mated with eight-week-old male C57BL/6J mice and continued to be fed HFD. There was no significant difference in pregnancy rate and number of pups between the mice fed with HFD and the mice fed with RD. In contrast, the mice fed with HFD tended to abandon the offspring and the survival rate of offspring born from the mice fed with HFD was significantly lower than that of offspring born from the mice fed with RD. These results suggest that HFD intake impairs psychological functions in female mice. Thus, we examined whether mice fed with HFD for 28 days show impairment of social behavior using social interaction test. The body weight of the mice fed with HFD was significantly increased compared to that of the mice fed with RD. In the three-chambered social interaction test, the social novelty preference of the mice fed with HFD was impaired. Taken together, our results suggest that HFD intake impairs social behavior in female mice, which disturb nurturing behavior.