Eukaryotic elongation factor (eEF2) kinase (eEF2K) that phosphorylates a specific substate, eEF2, suppresses protein translation. We previously demonstrated that acute intravenous injection of a selective eEF2K inhibitor, A484954, lowered blood pressure (BP) via affecting peripheral vasculature in spontaneously hypertensive rats (SHR). It is recently recognized that the dysfunction of release and sensitivity of neurotransmitters in brain mediates systemic hypertension. In the present study, in order to test the hypothesis that eEF2K in brain is involved in an increase in BP of SHR, we examined the effects of acute intracerebroventricular (i.c.v.) injection of A484954 on BP in SHR. A484954 lowered mean BP, suggesting that eEF2K in the brain might be involved in the increase in systemic BP in SHR.