Methamphetamine is one of the most abused drug in Japan and produces a strong rewarding effect. However, little is known about the mechanisms underlying methamphetamine-induced rewarding effect. Recent studies demonstrated that microRNA have important role in the regulation of several physiological functions. In the present study, we investigated the expression of microRNAs in methamphetamine-induced rewarding effect. The rewarding effect was evaluated by conditioned place preference. Methamphetamine (1mg/kg, s.c.) produced a significant rewarding effect. The mice were killed by decapitation and the limbic forebrain (containing nucleus accumbens) was dissected. Comprehensive analysis of microRNA expression in methamphetamine-induced rewarding effect was performed by microRNA array. The microRNA array analysis showed 20 significant changed microRNAs in the methamphetamine-induced rewarding effect. Especially, miR-7020-5p highly upregulated (fold change >30) in methamphetamine-induced rewarding effect. We next investigated the target scan for miR-7020-5p by database scan. We detect transient receptor protein subfamily C member 4 associated protein as a candidate gene. In conclusion, our findings suggest that the upregulation of miR-7020-5p may associate with the development of methamphetamine-induced rewarding effect.