Brain pericytes play an important role in the stabilization of vascular formation, maintaining the BBB function, regulating the blood flow synergistic with neuronal activation, and inflammatory processes. Hence, pericytes dysfunction may lead to numerous CNS diseases including neurodegeneration and cerebrovascular diseases. The aim of this study was to elucidate the role of pericytes in cerebrovascular disease. We isolated pericytes from Stroke-prone spontaneously hypertensive rat (SHRSP), which is accepted as an animal model of hypertension and cerebrovascular disease, and normotensive control rat (WKY). To reveal the role of pericytes on the function of the blood-brain barrier (BBB), we constructed an in vitro BBB model using brain endothelial cells (BECs) and pericytes. The effect of pericyte on the barrier function of BECs was similar between SHRSP and WKY, but the time course was different. We also performed the comparison of gene expression in pericytes between SHRSP and WKY.　Expression levels of several genes in SHRSP were different from that of WKY. Especially, analysis of gene expression comparison indicated that calcium responses might be changed in SHRSP. SHRSP pericytes might be involved in occurs BBB dysfunction even if chronic hypertension and onset of stroke are not well-established.