Sudden cardio death (SCD) is a sudden, unexpected death caused by loss of heart function. The cause of SCD is not well understood, and there is no effective medicine. GPx4 is the antioxidant enzyme which reduces the oxidized phospholipid using glutathione. We recently established new sudden cardio death mouse model with lipid peroxidation in heart GPx4 KO mice (KO mice) induced by change of vitamin E diet to normal diet. Next we screened the inhibitor of sudden cardio death using this mouse model and found that drinking of antibiotic cefoperazone (CPZ) in water completely could suppress the sudden cardio death in mice without direct antioxidant activity, but not the abdominal cavity dosage. To clarify whether CPZ resistant gut bacteria could suppress the lipid peroxidation dependent sudden cardio death in mice or not, we generated the germ free KO mice and a para-sterility KO mouse pretreated by four antibiotics. These KO mice drinking CPZ could not rescue sudden cardio death induced by decrease of the content of vitamin E in diet, indicating that CPZ resistant gut bacteria could suppress sudden cardio death in mice. We next examined the changes of enterobacterial flora by treatment of CPZ using the next generation sequencer. We found that CPZ treatment dramatically changed enterobacterial flora to almost only one bacteria in mice. This bacteria transplanted KO mice could rescue the cardio sudden death in mice. From these results, we first identified one enterobacteria that could suppress the sudden cardio death with lipid peroxidation in mice.