Emerging evidence suggests that astrocytic GqPCR signaling play an important role in animal behavior such as cognition, learning and memory. It has been shown that impairments of GqPCR-mediated Ca²⁺ signaling in astrocytes and/or gliotransmitter release derived from astrocytes in the hippocampus and/or cortex cause depression-like behaviors in mice. However, it is not clear whether astrocytic GqPCR signaling is a target for driving behavior related to anxiety and motivation. We focused on the ventral hippocampus (vHIP), which contributes to anxiety and depression, and investigated the effect of chemogenetic manipulation of astrocytic GpPCR signaling in innate behaviors related to anxiety or depression. We expressed hM3Dq, a Gq-DREADD, selectively in astrocytes in the vHIP by injecting AAV bilaterally. In acute slices, Gq-DREADD activation caused robust Ca²⁺ signals in vHIP astrocytes. Gq-DREADD activation of vHIP astrocytes increased travel distance in the center of the open filed without affecting total travel distance and travel velocity, suggesting that GqPCR activation in vHIP astrocytes may be anxiolytic. Gq-DREADD activation of vHIP astrocytes did not affect immobility time in tail suspension test. The data suggest that astrocytes in vHIP play an important role in innate behavior which is related to anxiety.