There are several methods for intratracheal administration using rodents.
Verified the oropharyngeal suction method and the micro spray method with the following contents.
2% Evans Blue was intratracheally administered by each method under anesthesia with isoflurane, and the distribution of lungs was confirmed.
Mice in which LPS-induced inflammation was induced in the same manner were also prepared. (Acute Lung Injury: ALI model)
BALF was collected 48 hours after LPS administration, and WBC, neutrophils, and monocyte/macrophages were confirmed.
And histopathological examination was also performed.
As described above, introduce the influence of differences in intratracheal administration methods on the phenotype in the acute phase.