Aim: We focused on eosinophilic esophagitis (EoE), a refractory rare disease, and tried to find the feature of oral microbiome in EoE patients and relationship between infection of pathogenic oral bacteria and EoE.
Method: Fifty healthy control volunteers, 52 EoE subjects were recruited. Bacterial DNA was extracted from the mouse-wash of subjects. Then, four popular periotontal pathogens (P.g, Prevotella intermedia (P.i), Tannerella forsythia (T.f), Treponema denticola (T.d)) and one caries-causing bacteria (S.m) were detected by PCR with specific paired-primer for each bacterium. Oral microbiome was analyzed by new generation sequencing. Furthermore, we examined correlationships between population or infection of bacteria and EoE-related parameters including number of eosinophils in esophagus, percentage of eosinophils in peripheral blood and serum IgE value.
Resutls: Infection of T.f and S.m were strongly correlated with EoE. Diversity of oral bacteria was smaller in EoE compared to that in healthy control. Only population of Neiserria mucosa was negatively correlated with number of eosinophils in esophagus although we examined all combination.
Conclusion: EoE subjects seems to have less abundant microbiome and higher infection rate of T.f and S.m. These changes possibly affect disease state of EoE.