Whereas virgin male mice show biting attack toward pup, mice who experienced mating and delivery of partner female show paternal behavior. The medial preoptic area (MPOA) is one of the most important area to control the behavioral pattern, attack or paternal. Our previous data suggest that the GABAergic synaptic inputs into the MPOA, presumably from medial amygdala (Me), were suppressed in the male with experience of mating and cohousing with pregnant female (father mice in gestation experience; FGE mice). However, it is not clear which components of “experiences with female” have effects to initiate plastic changes at the synaptic inputs into the MPOA. To address the effects of pregnant female exposure, virgin male stayed with pregnant female for 3 days. Then, we prepared brain slices derived from these mice and performed whole-cell patch-clamp recording from MPOA neurons. The amplitude of electrically evoked inhibitory post-synaptic potentials (eIPSP) was not different between virgin and virgin without mating experience. Next, we addressed the possibility that plastic changes in the MPOA was initiated by mating without cohousing until late pregnancy. The male mice were cohoused with partner female for 4 days and then isolated. After partner female got late pregnant, we obtained brain slices from this male and compared eIPSP amplitude. However, the eIPSP amplitude was not different compared with virgin male mice. We concluded that both mating and cohabitation until late pregnancy are required to induce female experience -dependent changes of inhibitory synaptic input in the MPOA of male mice.