Background: Artificial dialysis is divided into peritoneal dialysis (PD) and hemodialysis. Although PD provides higher quality of life than hemodialysis, few patients choose PD because of its inevitable dangerous. Long term PD evokes peritoneal deterioration which in turn triggers fibrosis and injured intestinal transit. It is necessary to discover the novel prevention target of peritoneal deterioration, but there were few reports because of the lack of clinical mouse model.
Methods: Peritoneal deterioration model mice were produced by methylglyoxal (MGO). After producing the models, the thickness of fibrotic layer and the capacity of intestinal transit were detected. The mRNA expression and protein level of fibrosis-promoting factors in peritoneal cavity were also measured.
Results: Peritoneum fibrosis and intestinal transit were aggravated with MGO treatment in time-dependent manner. Moreover, the mRNA expression of factor X was notably up-regulated in peritoneal cells. Protein level of factor X in peritoneal lavage fluid was also up-regulated in time dependent manner.
Conclusion: In this study, we confirmed that MGO peritoneal deterioration model showed  the pathology seen in clinical site. Moreover, we revealed that the production of factor X was up-regulated in accordance with the severity of pathology. Therefore, factor X might be the new prevention target of peritoneal deterioration caused by PD.