This study examines the effects of transmembrane protein 182 (TMEM182), which is highly expressed specifically in muscle tissue, on muscle differentiation. Although it is known that TMEM182 is involved in muscle differentiation, the detail is not fully elucidated. Therefore, we generated mouse skeletal myoblast cell line, C2C12, overexpressing TMEM182 in a doxycycline (DOX) -inducible manner. The results showed that the DOX-treated C2C12 inhibited differentiation into myotubular cells. To determine the mechanism, we are now searching for TMEM182 binding proteins by mass spectrometry. Because iPS cells do not express TMEM182 without differentiation, we are comparing undifferenciated iPS cells with the cells overexpressing TMEM182. We are also trying to investigate the effect of TMEM182 on myocardial differentiation using iPS cell-derived cardiomyocytes (iPS-CM) overexpressing TMEM182. As a result, the expressions of Nkx2.5, a cardiac progenitor cell marker, and TNNT2, a cardiomyocyte marker, were both decreased in iPS-CM overexpressing TMEM182 compared to control iPS-CM. These data suggest that TMEM182 regulates the differentiation of myoblasts into myotubes not only in skeletal muscle but also in cardiac muscle.