The desmosome is an intercellular junctional structure that provides strong adhesive forces between cells. Desmoplakin, an integral core protein of the desmosome, is expressed not only in epithelium and cardiac muscle but also in hippocampal neurons, which do not have desmosomes. The desmoplakin and the plakoglobin in cultured hippocampal neurons is shown to be coimmunoprecipitated with N-cadherin. However, there is still little evidence for the localization or function of desmoplakin in hippocampal neurons. Here, we found that three variants of Desmoplakin are expressed in the hippocampus. One of them corresponded to Desmoplakin Ia, which has only been identified in humans. We also confirmed that Desmoplakin mRNA is expressed in mature granule cells in the dentate gyrus of the mouse hippocampus by in situ hybridization. Desmoplakin immunoreactivity was localized to ciliary structures and dendrites of the granule cells. Exploration of the specific function of Desmoplakin in the granule cells of dentate gyrus may provide insight into the architecture and plasticity of hippocampal neural network.