Parkinson's disease (PD) is a progressive neurodegenerative disease that exhibits motor dysfunction due to deficit of dopaminergic neurons in the substantia nigra. Oxidative stress is a factor involved in the onset and progression of PD. We have previously shown that Actinidia arguta has an antioxidative activity. In this study, we evaluated the effect of prophylactic administration of Actinidia arguta (Sarunashi) in MPTP induced PD mice model. Male C57BL/6J mice were allowed to continuously ingest water or Sarunashi juice from 7 weeks of age. At 8 weeks of age, MPTP (30 mg/kg/day) was intraperitoneally administered for 5 consecutive days. In the MPTP + water group, significant motor dysfunction was observed in the pole test, catalepsy test, beam walk test, and rotor rod test compared with the negative control (NC) group. However, in the MPTP + Sarunashi group, no impaired motor function was observed as compared with the NC group. The protein expression level of tyrosine hydroxylase (TH) in the MPTP + water group was lower than that in the NC group. On the other hand, TH expression in the MPTP + Sarunashi group was higher than that in the MPTP + water group. In conclusion, prophylactic administration of Sarunashi juice suppressed MPTP induced motor dysfunction mediated via decreased TH expression.