The number of patients suffering from psychiatric disorders is extremely high worldwide. It has been identified that genes encoding voltage-gated calcium channels (VGCCs) subunits are associated with psychiatric disorders such as schizophrenia. The functions of VGCCs have been elucidated at the molecular level, whereas their roles in higher brain functions are largely unknown. In this study, we generated a forebrain glutamatergic neurons-specific VGCC β₄ subunit knock-out mice and conducted a comprehensive behavioral test. β₄ conditional knock-out mice showed altered sociability in three-chamber social interaction test. These knock-out mice also exhibited increased startle response. Therefore, this VGCC β₄ subunit conditional knock-out mouse may become a useful model for studying the psychiatric disorders related to sociability or auditory function.