Purpose
Fructose 1,6-bisphosphate (FBP) is a metabolic intermediate of glycolysis. Previous studies reported that FBP is preventive against oxidative stress. Thus we examined direct free radical scavenging activity of FBP.
Methods
Direct scavenging activity of FBP was evaluated against nine species of free radicals by electron spin resonance spectroscopy with the spin-trapping method. Cellular viability under oxidative stress was assessed by fluorescence-based assay using Alamar Blue.
Results
FBP significantly scavenged the following free radicals in dose-dependent manners; hydroxyl radical, superoxide anion, t-butoxyl radical, ascorbyl free radical, singlet oxygen, nitric oxide, and tyrosyl radical. However, no scavenging activity was observed against t-butyl peroxyl radical and DPPH. Cellular viability of MRC-5 cells exposed to hydrogen peroxide or antimycin-A was not improved in the presence of 0.1 μM –100 μM FBP. FBP showed no cytotoxicity up to 100 μM.
Conclusions
It was confirmed that FBP directly scavenges multiple free radicals including hydroxyl radical, superoxide anion and nitric oxide, indicating antioxidative activity through inhibition of the chain reaction in ischemia/reperfusion injury. Scavenging activity against tyrosyl radical might suggest inhibitive effect of FBP on COX reaction in inflammation.