Cerebrospinal fluid (CSF), a clear liquid produced in choroid plexus, is suggested to indicate physiological functions in brain. In the field of the central nervous system, CSF enables us to analysis brain pharmacokinetic parameters, predictive biomarkers, and changes in neurotransmitter. Therefore, CSF sampling technique is one of the indispensable tools for the pharmacological evaluation. To provide enough support for researchers, we have been working on building useful methods for collecting CSF from the cisterna magna of small animals such as rats or mice. In this presentation, we focus on short-term sampling methods using rat (multiple times per day), or long-term sampling methods using mice (multiple times per month). In addition, we would like to introduce our recent efforts for a stereotaxic injection system, an intrathecal injection system, and an in vivo brain microdialysis technique.