H₂S, a gasotransmitter, is enzymatically formed from L-cysteine. We have reported that intraplantar injection of H₂S donors, NaHS and Na₂S, causes hyperalgesia/allodynia in mice, an effect abolished by genetic deletion of Ca_v3.2 among three isoforms of T-type Ca²⁺ channels (T-channels). Interestingly, their intradermal (i.d.) injection in mouse cheek simultaneously produces itch and pain, which are reduced by pharmacological blockade of T-channels. In the present study, we tested if distinct sulfides would induce itch and pain in the mouse cheek i.d. injection model, and examined the effect of Ca_v3.2 genetic deletion. The itch and pain responses, i.e. scratching with a hindlimb and wiping with a forelimb, respectively, were counted for 60 min following i.d. injection of test compounds in mice. Na₂S and Na₂S₄ at 30-300 µg (0.38-3.8 and 0.17-1.7 µmol, respectively) as well as L-cysteine at 121-6050 µg (1-50 µmol) caused both scratching and wiping behaviors in a dose-dependent manner. The Na₂S-induced scratching and wiping were abolished by TTA-A2, a selective T-channel blocker, and by genetic deletion of Ca_v3.2. Our study thus demonstrates that sulfides including L-cysteine as well as polysulfides participate in the T-channel-dependent itch and pain, and provides definitive evidence for the role of Ca_v3.2 in the sulfide-induced itch and pain.