Neovascular glaucoma is caused by the formation of new blood vessels in cornea and iris of retinal ischemic disease, such as retinal vein occlusion (RVO), which can reduce the visual acuity. However, the effect of neovascular glaucoma is still not well undertood because there is no model with neovasular glaucoma. The purpose of this study was to investigate the involvement of neovascular glaucoma and ischemic disease by using murine RVO model with neovascular glaucoma. Here, we investigated time-dependent changes of fibrosis-related factors and the intraocular pressure in this model. Moreover, we performed vascular endothelial growth factor (VEGF) level in the retina and cornea using qRT-PCR. The fibrosis-related factors were increased in the retina. The intraocular pressure was elevated in mice with neovascular glaucoma. Interestingly, the expression of VEGF was not increased in the retina, but increased in the cornea. These findings indicate that neovascular glaucoma in the retinal vascular disease may be associated with the fibrosis-related factors.