Pneumonia is recognized to be a more serious disease by the spreads such as recent COVID-19 infectious diseases.
It is thought that treatment for the acute pneumonia in particular is important in pneumonia.
In generally, LPS-induced ARI animal model are used for the study.
Therefore, we made two kinds of pulmonary inflammatory models using the LPS and evaluated and compared them.
One is a model to let LPS expose to a whole body, and another one is instillation of LPS to nasal cavity under anesthesia with a pretreatment of α-garactosyl ceramid (αGalCer).
These two animal model was evaluated inflammation cells infiltration into lung by BALF and cytokines in BALF supernatant by FCM.  
And evaluation by drug was carried out oral administration of dexamethasone.
As a result, it was revealed that two models were different in a characteristic each.
Based upon these results, we confirmed that these two ARI model induced by LPS was the model which could carry out the evaluation of the test article in consideration of a characteristic each.
At the commencement of this study, the study protocol was reviewed by the Institutional Animal Care and Use Committee of the test facility and approved by the general manager of the Nonclinical Research Center according to the following guideline: Guidelines for Animal Studies (Nonclinical Research Center, LSI Medience Corporation).