Folate is essential for normal fetal development and growth. Our previous study has described that prenatal folate deficiency (FD) mice have decreased sociability and impaired memory and recognition. Enriched environment (EE) is known to positively influence emotional and cognitive functions. In this study, we investigated whether EE could improve behavioral impairment in FD mice. ICR mice were fed a control diet of chow (2 mg folate/kg diet) or a folate deficiency diet of chow (0.3 mg folate/kg diet) from embryonic day 1 to delivery. The mice were weaned on postnatal day 21 and were housed under either standard environment (SE) or EE. We evaluated the function of sociability and memory/recognition at age 7-10 weeks. The decreased sociability was improved in FD mice by EE in the 3-chambered social test. However, the impaired functions of spatial memory in Morris water maze test and object recognition memory in novel object recognition test were not improved by EE in FD mice. Next, we examined the changes in the relative expression of GABA related proteins in prefrontal cortex (PFC). The expression of GABA-synthesizing enzymes (GAD65/67) significantly decreased in FD mice compared with control mice. However, GAD65/67 levels did not differ between mice reared in SE and EE. These results may suggest that FD induces behavioral impairment and decrease in GABAergic system and that EE reverts decreased sociability but not impaired function of memory and recognition in the FD mice without changing the expression of GAD65/67 in the PFC.