Glycerophospholipids (PLs) play essential roles in our body, such as main structural components of biological membranes and precursors of bioactive lipid mediators. Cumulative evidence indicates that, in some cases, abnormality of PLs synthesis may result in embryonic or neonatally lethality in mice. Therefore, “Quality of Lipid” is considered as a critical aspect to influence the cellular functions. Recently, we attempt to evaluate quality of lipid in the dorsal root ganglion (DRG), and examine how it is regulated and how it causes sensory transmission (touch, heat and cold, pain, itch, and chemical stimuli). We revealed the diverse expression pattern of biosynthetic enzymes of PLs (lysophospholipid acyltransferases) in the mouse DRG. Moreover, PL compositions and production of lipid mediators in the DRG were altered by peripheral nerve injury. These results suggest that understanding of “Quality of Lipid” may lead to unveil the regulatory mechanisms of sensory transmission in both physiological and pathological conditions.