Mushrooms are known to be abundant biological resources because millions of them are presumed to exist all over the world. Among them, not only edible but pharmaceutical mushrooms have been identified and utilized. However, many of unidentified mushrooms and their factors remain yet. Tottori University organizes Fungus/Mushroom Resource and Research Center (FMRC) which is challenging to realize the infinite potentials of mushrooms as the generic biological resources through various studies from basic to applied subjects using Tottori University Fungal Culture Collection (TUFC). FMRC is promoting to collect and banks the mushroom extracts for exploring the biological and pharmaceutical usages. In this study, to clarify the activity of anti-cancer, we investigated effects of mushroom extracts on the viability of cancer cell lines. The cell viability was measured by using a WST-8 reagent and a trypan blue dye exclusion test. The apoptotic cells were detected by annexin V binding assay. The production of reactive oxygen species (ROS) was detected by using H₂DCFDA, a fluorescent indicator. At first, effects of extracts from dozens of myceliums and their filtrates on the cell viability were examined. Several samples showed stronger cytotoxic action in cancer cell lines than normal ones. An analytical chemistry technique succeeded to identify a few compounds from their extracts. They induced the apoptosis without the generation of ROS. We found that some mushroom extracts banked in FMRC may be useful as biological resources for the development of anti-cancer reagents.