Recent advances in high-throughput technologies have facilitated the collection of multilevel omics data. It has driven growth in public databases and in the development of bioinformatics tools. These resources can be used to generate disease-related biological networks and to identify potential therapeutic targets within the networks. The potential therapeutic targets identified by such integrated approaches, however, still need to be validated using in vitro and/or in vivo disease models. Zebrafish can be powerful tools in the drug discovery field, given advantages such as high fecundity, ease of drug administration and genetic manipulation, and similarity to mammals in terms of structures and functions of various tissues. In this session, I would like to demonstrate our approach utilizing omics technology and zebrafish to discover potential therapeutic targets for various disorders.