Purpose: H. pylori is a major cause of gastritis and gastric ulcer. In recent years, it has been reported that probiotic has an inhibitory effect on H. pylori colonization. MAX-PROBIO, a probiotic product, contains 44 strains such as lactic acid bacteria and bifidobacteria. In this study, in vitro and in vivo experiments were performed to assess the inhibitory effect of MAX-PROBIO on H. pylori.
Methods: In the in vitro experiment, MAX-PROBIO was added to Brucella broth adjusted to pH 3, and the mixture was incubated. H. pylori cultures were incubated under microaerophilic conditions at 37°C with the broth culture supernatant. The viability of H. pylori after incubation was assessed by counting the number of viable colonies. In the in vivo experiment, H. pylori was inoculated orally to mice (C57BL/6JJms Slc). MAX-PROBIO was then orally administered to the H. pylori-infected mice for 4 weeks, and the number of viable H. pylori in the stomach was counted.
Results and Conclusion: The number of viable H. pylori was significantly decreased in the culture supernatant cultured (in vitro) and in the stomach of MAX-PROBIO-administered mice (in vivo). These findings revealed that MAX-PROBIO contributes to suppression of growth of H. pylori.