RNA virus infections induce exacerbation and increase the morbidity of patients with chronic airway diseases. We have previously demonstrated that polyinosinic-polycytidylic acid (poly(I:C)), a TLR3 agonist, induced corticosteroid insensitive airway inflammation. The aim of this study is to evaluate the effects of Src inhibitor on poly(I:C)-induced corticosteroid insensitive airway inflammation in mice. A/J mice were exposed with poly(I:C) intranasally twice daily for 3 days. Dasatinib (Das; Src inhibitor) were administered intranasally at 2h before each poly(I:C) exposure. BALF was collected at 24 h after the last poly(I:C) exposure and neutrophils were quantified by FACS analysis. The level of CXCL1, TNF-α and osteopontin (OPN) in BALF was determined using a commercially available kit. Poly(I:C) exposure showed significant increase in neutrophils CXCL1 in BALF. Das showed dose-dependent inhibition of airway neutrophilia and CXCL1 production in poly(I:C)-exposed mice. In addition, Das inhibited TNF-α and OPN production induced by poly(I:C) exposure at higher dose. This profile provides new insights into corticosteroid insensitive airway inflammation induced by virus infection and future treatment.

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