Local metabolite and ionic strength are important factors for maintaining the functions of living cells. We have developed micro-nanoscale electrode and electrochemical sensor based scanning probe microscopy to measure the spatial electrochemical metabolite and ion concentration profile near the sample surface with nanoscale resolution. Scanning electrochemical microscopy (SECM) uses an ultramicroelectrode as a probe for detecting electroactive chemical species (oxygen, ATP, and reactive oxygen species (ROS), and neurotransmitter). SECM has been recognized as an effective tool for investigating micrometer-scale local chemical flux. Miniaturization of the electrode is an important factor for improving SECM resolution. Electrode–sample distance control is also an important factor for measuring fast chemical flux and improving SECM resolution. Distance control by ion current feedback is a promising way for the non-contact investigation of soft materials, SECM–scanning ion-conductance microscopy (SICM) has been used in a hybrid system to improve SECM resolution by controlling the electrode probe and sample distance in solutions without direct contact. SICM is also useful for detecting the ion concentration profile and charge measurement in a solution. We measured the 3D chemical and ion current using SECM–SICM. In this presentation, we report the SICM topography images of gastric surface mucous cell lines (GSM06), which produce periodic acid-schiff and concanavalin A positive glycoproteins. To visualize the damage process of the mucosal layer, we added ethanol to GSM06 cells and imaged the topography change using SICM. We also performed topography and electrochemical simultaneous imaging using SICM-SECM to identify the mucosal layer without labelling.