It has been reported that the administration of butyrate, a short-chain fatty acid produced by microbiota in the intestine, induces expression of the genes involved in peroxisomal fatty acid b-oxidation. In the present study, we investigated whether administration of the butyrate-producing probiotics (Clostridium butyricum MIYARI 588, MYR) inhibit the progression of obesity and studied the effect of added dietary fiber and the mechanism in lipid metabolism. Male C57BL6J-ob/ob mice were divided into 3 groups, Control, MYR and inulin (INU) groups. Animals in MYR and INU groups were fed diet containing 3% MIYARI 588 ad libitum. Drinking water for INU group were added 1% inulin. Last week of the 7-week administration period, oral glucose-tolerance test was performed. The relative weights of liver and white adipose tissues (WAT) in MYR and INU groups were lower than Control group. Compared with Control group, Adiponectin expression in WAT of MYR group was tended to be higher, and that of MYR group was higher. In conclusion, it is suggested that the 7-week administration of MYR induces the changes in lipid metabolism involved in adiponectin expression in WAT accompanied by the decreases in liver and WAT weight.