Acute kidney injury (AKI) represents a major clinical problem with high mortality in kidney transplantation and nephron-sparing surgery. The ischemic AKI model by the interception of the bloodstream is the superior model that can evaluate an efficacy evaluation and condition of a patient elucidation in a short term for AKI without the therapeutic drug. We reported that agmatine has the preventive effect on ischemic AKI. But we do not find the detailed mechanism about the renoprotective effect of agmatine. Therefore, we examined the detailed renoprotective mechanism of agmatine with the high coverage expression profiling (HiCEP). Male Crl:CD1 mice's right kidney was removed. After a 2-week, to induce ischemic AKI, the left renal artery and vein were occluded with a clamp for 50 min. Agmatine was injected 5 min before the ischemia. At 6 h after reperfusion, we excised the left kidney and detected the gene change with HiCEP. As a result, we obtained the gene changes of TRXR, synbindin, ADAMTS1 and PEA15.