Uric acid (UA) is well known as the end product of purine metabolism in human. Hyperuricemia is defined as a serum UA level of ≥ 7.0 mg/dl (416 µM) in Japanese guideline for the management of hyperuricemia and gout (second edition), which is known as a risk factor for cardiovascular disease. Recently, it has been reported that low UA levels as well as high UA levels are predictive markers of increased mortality in epidemiologic studies. Hence, the aim of this study is to assess the role of UA at physiological concentration (conc) in normal cells. Normal human dermal fibroblasts were treated with UA conc at 62.5 - 500 µM (1.1 - 8.4 mg/dl). Cell viability of UA treated groups within the normal range was higher than that of control group. There was no change in cell number among groups, but total protein levels in UA treated groups increased at 72 hours. On the other hand, UA is known to have the potential antioxidant effects. Physiological conc of UA treatment decreased reactive oxygen species in the present study. Additionally, the expression of an oxidative stress-related protein was increased by UA treatment. Taken together, these findings suggest that physiological conc of UA in normal cells is probably implicated in cell viability.