Recent advances in super-resolution microscopy has enabled diffraction-unlimited imaging of cellular structures and protein distributions in living cells. Many commercially available microscopes are now equipped with super-resolution mode, however, knowledge about how to choose an appropriate microscopy for each experiment is not shared among researchers yet. In this session we will introduce various types of state-of-art super-resolution microscopes with their actual applications and discuss about some critical points to notice when choosing microscopes for live-cell super-resolution imaging. We would also talk about our recent development of a novel live-cell RNA imaging tool and its application with super-resolution microscopy.