Recent advances in super-resolution microscopy have overcome a limitation of spatial resolution in fluorescence imaging imposed by the diffraction of light, enabling nanoscale molecular imaging with conventional fluorescent probes. Single-molecule localization based super-resolution microscopy, including stochastic optical reconstruction microscopy (STORM), provides multi-color and three-dimensional imaging with a remarkable spatial resolution, and thus resolves detailed nanostructures in biological specimens. Fortunately, it can be performed with a simple wide-field microscope setup and is completely compatible with immunohistochemical fluorescent staining methods. In this seminar, I will talk a detailed method for STORM imaging, from sample preparation to image analysis, with some tips on how to improve the spatial resolution. I will then show some examples for STORM imaging of immunohistochemical specimens, including the brain and pancreas.