Virus infection is generally associated with virus-driven hijacking of host cellular machineries including translational regulation. Host shutoff is known as a strategy used by viruses to repress cellular mRNA translation and parallelly allow the efficient translation of viral mRNA. Host shutoff could be achieved by two complementary mechanisms: either direct co-opting of the translation machinery that forces better translation of viral mRNAs compared to their host counterparts, or viral-induced degradation of host mRNAs. However, it remains unclear how host shutoff and viral mRNA translation is regulated upon influenza virus infection. In the present study, to systemically analyze the dynamic changes in host and viral mRNA translation machinery, we performed ribosome profiling and RNA sequencing (RNA-seq) analysis in the mouse embryonic fibroblasts infected with influenza A (H1N1/PR8) virus. We will report the global profiles and characteristics of gene expression and translational states for host and virus mRNA during the course of influenza infection, which could lead to a better understanding of mRNA translation machinery in influenza virus infection.