Toxicogenomics project is a Japanese government and pharmaceutical companies joint project to gather the gene expression data after exposure to about 150 compounds to rats in vivo for up to 28 days, rats and humans in vitro. The data are available for public use on the internet as "Open TG-GATES". Analysing the microarray data from "Open TG-GATES", genes fall in the following criteria were selected 1) increase more than 2 times, 2) the expression changes occur within 7 days from the initial dose, 3) those changes occur with several carcinogenic agents, 4) not or minimally expressed in the normal liver, and 5) related to proliferation or apoptosis. Several genes fell in the criteria and we focused on one of them, p75-NTR associated cell death executor (NADE) gene. NADE is known to bind to some partner proteins in the cell and in most cases, cells die by apoptosis. We further investigated whether NADE is a promising early carcinogenic marker using carcinogen exposed rats. Sprague-Dawley rats were treated with diethylnitrosamine for up to 8 weeks followed by up to another 11 weeks of no treatment. The liver was excised to obtain RNA and protein samples and paraffin embedded tissue sections. The NADE expression pattern correlates with the development and growth of cancerous cells. It is likely that the NADE plays a role in chemically induced carcinogenesis in the rat liver and it can be a good early gene marker of carcinogen.