Multi-electrode array (MEA) assays using human induced pluripotent stem cell (hiPSC)-derived neurons are expected to predict the convulsion toxicity of new drugs. In the reliability of the evaluation system, it is important to compare the response of hiPSC-neuron with the response in the living brain. In this study, we compared the responses to typical convulsants in cultured hiPSC-derived cortical neuronal network compared with mouse acute hippocampal slice. 4-Aminopyridine (4-AP), Pilocarpine, Picrotoxin(PTX), Pentylenetetrazol (PTZ), and negative control acetaminophen, DMSO were tested in both samples using MEA system. The change of number of burst, inter burst interval (IBI) and CV of IBI in negative control and DMSO administration were almost same both samples. Although the number of burst increased both sample in convulsants administration, dose-dependency were difference between hiPSC-derived neurons and acute slices. However, CV of IBI were decreased dose-dependently both samples. In three convulsants, it was found that the hiPS neuron and the acute slice exhibit partially similar firing patterns.