It has been reported recently that there is a possibility that accumulation of αsynuclein causes neurological problems and introduces central nerve system disorders. Most of the studies have been performed using genetically-modified animals or animals prepared with virus vectors. These animal models are not suitable for efficacy screening of drugs.
The present study was performed to establish an in vivo screening model which can be used easily for drug efficacy evaluation of cytotoxicity of αsynuclein.
[Methods] An αsynuclein was administered to the lateral ventricle of male mice at 6 weeks of age. Behavioral assessment was performed 7 to 12 days after administration by the Y-maze test and passive avoidance test.
[Results] The αsynuclein solution administered to the lateral ventricle impaired short-term memories. The animals recovered from the impairment by donepezil administration.
[Compilation] A mouse model whose lateral ventricle was treated once with the αsynuclein had impaired learning. This impairment was improved by donepezil. Therefore, the possibility is suggested that this mouse model can be an in vivo screening model which can be used easily in a short period for drug efficacy evaluation of cytotoxicity of αsynuclein.